exam questions on chromatography

What useful information can be found from a Van Deemter plot? Answer. C. adsorption chromatography The dipeptide cysteine-aspartic acid (cys-asp), J, and the dipeptide aspartic acid-cysteine (asp-cys), K, are shown mixture of two dipeptides J and K analysed by gas chromatography followed by mass spectrometry (GC-MS). Scores are calculated assuming a maximum score of 100 pts. B. V = V0/Vi d) Ascending – descending chromatography. Answer. Chromatography is a physical method that is used to separate and analyse __________ a) Simple mixtures b) Complex mixtures c) Viscous mixtures d) Metals Answer: b Explanation: Chromatography is a physical method that is used to separate complex mixtures. In reverse phase chromatography, the stationary phase is made, A. non-polar 4. c) Radial paper chromatography Resolution is proportional to the square root of the number of theoretical plates in a column, Resolution is inverse proportional to the square root of the number of theoretical plates in a column, Resolution is proportional to the square of the number of theoretical plates in a column, Resolution is proportional to the number of theoretical plates in a column, Resolution is inverse proportional to the number of theoretical plates in a column, Clean an LC column from materials that deposited on the head of the column, Measure resistance to flow through a chromatographic system, Describe analyte envelope as it moves through the stationary phase, The distribution of an analyte between the stationary and the mobile phase, The migration rate of an analyte through a column, More polar compounds toward the top of the plate and less polar toward the bottom, More polar compounds toward the bottom of the plate and less polar toward the top, Lower boiling compounds toward the bottom of the plate and higher boiling toward the top, Lower boiling compounds toward the top of the plate and higher boiling toward the bottom, Determining the number of components in a mixture, Vapor pressure and polarity of the solute, More versatile in adjusting separation parameters, Compound identification through library matching, An adsorption process, results in an irreversible chemical reaction of the analyte with sorbent surface, An absorption process, results in an irreversible chemical reaction of the analyte with sorbent surface, An adsorption process, results in an reversible chemical reaction of the analyte with sorbent surface, An absorption process, results in an reversible chemical reaction of the analyte with sorbent surface, Removing question excerpt is a premium feature, which of the following is correct statement. a) D…, Analyzers Based on Gas Density Questions & Answers…, Analyzers Based on Gas Density Questions & Answers 1. Chromatography Exam. Explanation: In Column chromatography, the stationary phase is made of solid and the mobile phase is made of liquid. This is because chromatography is not performed under severe conditions. c) Viscous mixtures C. molecular weight What is chromatography ? [1] Sample question 2 - Foundation Question. B. conductivity A combination of paper chromatography and electrophoresis involves, A. partition chromatography 8. Explanation: In Gas-liquid phase chromatography, the stationary phase is made of liquid and the mobile phase is made of gas. b) Column The same liquid, paper and pen were used in each of the three experiments shown. 15. B. electrical mobility of the ionic species Explanation: In chromatography, the mobile phase can be composed of liquid or gas. d) Liquid only. Will travel slowly but will effectively separate components. Quiz. Question.14. Quiz: Are You A True Star Wars Series Fan. Explanation: In Radial paper chromatography, the mobile phase moves horizontally over a circular sheet of paper. When analyzing GC data what does the position of the peaks mean and the height of the peaks? 3. High flow rate is fast but less quality separation, low flow rate is bad because molecules flow back in. b) Liquid or gas 2012 - Higher Paper chromatography was used to find the composition of brown ink in a pen. 10. Sample Exam Questions 1. It is based on the differential migration of the individual components of a mixture through a – stationary phase under the influence of a moving phase. c) Liquid chromatography The mixture of different components is flushed through the system at different rates. 7. Explanation: Thin layer chromatography involves the separation of substances of a mixture over a 0.2mm thick layer (thin layer) of an adsorbent. Which of the following is true reg. a) Solid or liquid d) Metals. d) Liquid only. It cannot be a solid material. Explanation: In Planar Chromatography, stationary phase is supported on flat plate of paper. In gas chromatography, the basis for separation of the components of the volatile material is the difference in, A. partition coefficients Explain its working principle and what are its limitations? In which of the following type of paper, chromatography does the mobile phase move horizontally over a circular sheet of paper? It could be gas-solid chromatography or gas-liquid chromatography. b) Silica gel Module. c) Gas Chromatography c) Viscous mixtures As different fractions are eluted off the column the polarity of the eluent can be gradually increased, fractions are collected and labeled, TLC plates ran on fractions to test components of mixtures. d) Planar Chromatography, A. partition chromatography Flashcards. Match. What are some solvents good for hydrocarbons? Share. There are always two phases in chromatography, what are they? If several compounds are present in a sample which is developed on a TLC plate, a column of spots is seen on the developed plate, with: TLC is generally used as a qualitative analytical technique for. Use the following gas chromatogram of a mixture of dioxane and cyclohexane eluted on a non-polar column to answer the following two questions.

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